Anti-Mast Cell Tryptase Mouse mAb (AA1) (C15-1303-649)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.

Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Immunogen

Human

native, human mast cell, tryptase

Application

Frozen Sections (0.06-0.12 µg/ml)

Immunoblotting (1 µg/ml)

Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Cat, dog, human, or monkey mast cells

Other Notes

Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.

Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: AA1, monoclonal. form: liquid. does not contain: preservative. species reactivity: monkey, canine, human, feline. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG1. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... TPSAB1(7177). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.