ANTI-MECP2 CLONE 1L20 ZOOMAB RABBIT MONOCLONAL (C15-1671-039)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1L20 is a Rabbit recombinant monoclonal antibody that specifically detects Methyl-CpG-binding protein 2 (MeCP2). It targets an epitope within 14 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Methyl-CpG-binding protein 2 (MeCP2).

Application

Quality Control Testing

Evaluated by Western Blotting in Cos-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in Cos-7 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MeCP2 in MCF-7 and HeLa cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MeCP2 in A431, HeLa, HepG2, and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MeCP2 in human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound MeCP2 with a KD of 9.6 x 10-9 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MeCP2, clone 1L20 ZooMAb^®, Cat. No. ZRB1156, is a recombinant Rabbit monoclonal antibody that specifically targets MeCP2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Methyl-CpG-binding protein 2 (UniProt: P51608; also known as MeCp-2 protein, MeCP2) is encoded by the MECP2 gene (Gene ID: 4204) in human. MeCP2 is a chromosomal protein that binds to methylated DNA and is present in high abundance and frequency at its target sites. It can bind specifically to a single methyl-CpG pair and is considered as a reader of the DNA methylome. It is shown to binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). It contains a methyl-CpG binding domain (MBD; aa 90-162) that is responsible for its binding to DNA. MeCP2 mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. It is expressed in all tissues, but higher levels are present in neurons. Its levels increase during embryonic and postnatal development and then plateau off around the age of 10 years in humans. It can undergo multiple post-translational modifications at multiple sites by phosphorylation, ubiquitination, SUMOylation, acetylation, methylation, GlcNAcylation and poly(ADP-ribosyl)lations. These modifications affect its stability and functions. It is reported to be phosphorylated on serine 423 in brain upon synaptic activity that attenuates its repressor activity and regulates dendritic growth and spine maturation. Mutations leading to loss-of-function are known to cause Rett syndrome that leads to mental retardation. Most of these mutations have been found in the MBD region. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tillotson, R., and Bird, A. (2020). J. Mol. Biol. 432(6); 1602-1623).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.