Specificity
MEK 5 is also known as MAP Kinase Kinase 5, or ERK Kinase 5. AB3184 detects a 49 kDa protein, corresponding to the expeted molecular mass of Mek5, in rat brain, rat liver, rat kidney, rat heart, mouse brain, and mouse testis tissue extracts as well as in cell lysates prepared from the rat pheochromacytoma (PC12) and the human HeLa cell lines. No cross-reactivity with MEK 1, 2, 3, 4 or 6 has been observed with this antibody.
Immunogen
Human MEK 5 synthetic peptide (aa. 59-74; Genbank Accession # U71088), conjugated to KLH; identical to rat MEK5 sequence over these residues.
Application
Anti-MEK5 Antibody is an antibody against MEK5 for use in ELISA, IH, IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases
Western Blot: 1 μg/mL
Immunohistochemistry: 1:1,000
Immunoprecipitation
EIA
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified immunoglobulin. Affinity purified immunoglobulin
Liquid containing PBS, pH 7.2. No preservatives.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Thawed product may be stored at 2-8°C for up to 4 weeks. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: Xenopus, canine, rabbit, sheep, monkey, human, mouse, rat. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_002757.2, NM_145160.1. UniProt accession no.: Q13163. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... MAP2K5(5607)mouse ... Map2K5(23938). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 12352203
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- AB3184
- Temperature Control Device:
- Yes
