Anti-Melanin Concentrating Hormone Receptor Type 2 Antibody, intracellular, CT (C15-1316-383)

General description

Melanin Concentrating Hormone Receptor type 2 (MCH-R2) is a G-protein coupled receptor with seven transmembrane domains. MCH-R2 is 340 amino acids in length (approximately 39 kDa) and shares approximately 38% amino acid identity with MCH-R1. Like MCH-R1, it binds MCH with high affinity. However, reports indicate that signaling through this receptor is pertussis insensitive, contrary to MCH-R1. Expression of MCH-R2 mRNA is restricted to several regions of the brain, including the arcuate nucleus and the ventral medial hypothalamous (Sailer, 2001).

Specificity

Human Melanin Concentrating Hormone Receptor Type 2 (MCH-R2). The antibody has been used to stain tissues from rat and mouse as well as human.

Immunogen

Epitope: intracellular, C-terminus

Synthetic 16 amino acid peptide corresponding to the intracellular carboxy-terminus of human MHC-R2.

Application

Immunohistochemistry: 1 μg/mL on brain cryostat sections. The antibody stains extensively through the brain and is particularly striking in the ganglionic layer of the cerebellum.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Hormones & Receptors

This Anti-Melanin Concentrating Hormone Receptor Type 2 Antibody, intracellular, C-terminus is validated for use in IH for the detection of Melanin Concentrating Hormone Receptor Type 2.

Physical form

Affinity purified immunoglobulin in PBS, pH 7.2 containing 0.1% sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. The antibody can also be diluted 1:1 with glycerol (ACS grade or better) for added stability. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: chicken. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable. NCBI accession no.: NM_001040179.1, NM_032503.2. UniProt accession no.: Q969V1. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... MCHR2(84539). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.