ANTI-MERS-COV NUCLEOPROTEIN. CLONE 46 (C15-1886-704)

Specificity

Clone Lt-4 is a mouse monoclonal antibody that detects Protein Tax-1.

Immunogen

Partially purified Tax-1 protein preparation from HTLV-1 infected TCL-Kan cells

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human T-cell leukemia virus 1 Protein Tax-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Human T-cell leukemia virus 1 Protein Tax-1.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Protein Tax-1 in Flow Cytometry applications (Peres, C., et al. (2019). J Virol Methods. 274:113728).

ELISA: A representative lot detected Protein Tax-1 in ELISA applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein Tax-1 in Immunoprecipitation applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Western Blotting Analysis: A representative lot detected Protein Tax-1in Western Blotting applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11; Tanaka, Y., et al. (1991). Int J Cancer. 48(4):623-30).

Immunocytochemistry Analysis: A representative lot detected Protein Tax-1 in Immunocytochemistry applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein Tax-1 (UniProt: P03409; also known as Protein X-LOR, Protein PX, Trans-activating transcriptional regulatory protein of HTLV-1) is encoded by the Tax gene in human. Tax-1 is a homodimeric protein that serves as a transcriptional activator that governs the viral transcription from the 5′LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. It is reported to shuttle from the host nucleus to the cytoplasm and is found predominantly in the nucleus, where it is equally distributed between the nucleoplasm and the nuclear matrix. Tax-1 is reported to modulate the expression of cellular genes leading to the deregulation of T-cell proliferation, disturbing the integrity of cell cycle checkpoints, the DNA damage response, and apoptotic pathways. It is also shown to act as a ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of NF-kB. Phosphorylation of Tax-1 on threonine 48 is shown to result in the loss of NF-kB activation function. Clone Lt-4 recognizes the Tax-1 protein expressed in HTLV-I-infected cells but does not react with uninfected cell lines and fresh and PHA-activated peripheral blood lymphocytes. (Ref.: Peres, C., et al. (2019). J. Virol. Methods. 274; 113728; Wang C., et al. (2016). PLoS Pathog. 12(4); e1005584; Tie, F., et al (1996). J. Virol. 70(12); 8368-8374; Tanaka, Y., et al. (1991). Int J Cancer. 48(4); 623-630; Lee, B., et al. (1989). Tohoku J. Exp. Med. 157(1); 1-11).

Physical form

Purified mouse monoclonal antibod yIgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/ml. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.