ANTI-MERS-COV NUCLEOPROTEIN. CLONE 46 (C15-1886-707)

General description

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Clone R52 can detect both native spike protein expressed in HEK293T cells and the artificial spike glycoprotein carrying T4 foldon. It recognizes a continuous epitope in amino acids 549-568 of spike protein receptor binding domain. (Ref.: Guo, Y., et al. (2021). J. Biol. Chem. 296; 100346).

Specificity

Clone R52 is a mouse monoclonal antibody that detects SARS-CoV-2. It targets an epitope within the extracellular domain.

Immunogen

GST-tagged recombinant fragment corresponding to 171 amino acids from the S1 domain of SARS-CoV-2 spike protein expressed in E. coli.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 spike protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant SARS-CoV-2 spike protein.

Tested Applications

Western Blotting Analysis: A representative lot detected SARS-CoV-2 spike protein in Western Blotting applications (Guo, Y., et al. (2021). J. Biol. Chem. 296:100346).

ELISA Analysis: A representative lot detected SARS-CoV-2 spike protein in ELISA applications (Guo, Y., et al. (2021). J. Biol. Chem. 296:100346).

ELISA Analysis: Various dilutions of this antibody detected recombinant Spike glycoprotein of SARS-CoV-2.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 S1 domain, clone R52, Cat. No. MABF3061, is a mouse monoclonal antibody that detects Spike glycoprotein of SARS-CoV-2 and is tested for use in ELISA and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.