Specificity
Metabotropic glutamate receptors mGluR2 and mGluR3, which have nearly identical C-termini. There is also a very slight recognition of mGluR5. Western blot analysis of rat brain shows bands migrating at Mr = 100,000 (which contains both mGluR2 and mGluR3) and 190,000. The larger band is thought to be a dimer of the smaller band.
Immunogen
Carboxy terminus peptide of rat mGluR2 conjugated to BSA with glutaraldehyde (NGREVVDSTTSSL).
Application
Anti-Metabotropic Glutamate Receptor 2/3 Antibody detects level of Metabotropic Glutamate Receptor 2/3 & has been published & validated for use in IH(P), IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot analysis can be done at final concentrations of 0.1-0.5 μg/mL. AB1553 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.1-0.5 μg/mL.
The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.
Storage and Stability
Maintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, monkey. manufacturer/tradename: Chemicon®. . technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. NCBI accession no.: NM_000839.2. UniProt accession no.: Q14416. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: rat ... Grm2(24415)rhesus monkey ... Grm2(700214). Storage Class Code: 10 - Combustible liquids.
- UPC:
- 51402002
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1553
