Anti-methyl-CARM1 (Arg551) Antibody (C15-1317-155)

General description

CARM1, also known as Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4, and encoded by the gene name CARM1 and PRMT4, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. CARM1 methylates (mono- and asymmetric dimethylation) the the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Upon gene activation CARM1 together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ′Arg-17′ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. CARM1 is overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Immunogen

Epitope: Methylated R551

Linear peptide corresponding to human CARM1 (Arg551) methylated at R551.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated methyl-CARM1 (Arg551) in vitro methylation of a control buffer, 15 mg nuclear lysates, and 15 mg cytoplasmic lysates from CARM1/MEF cells by 1 mg CARM1 WT and CARM1 R551K (Kuhn, P., et al. (2011). Nucleic Acids Research. 39(7):2717-2726).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-methyl-CARM1 (Arg551) | ABE430 antibody is validated for use in WB, IP for the detection of methyl-CARM1 (Arg551) | ABE430.

Quality

Evaluated by Western Blotting in HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected methyl-CARM1 (Arg551) in 10 µg of HEK293T CARM1 KO cells restored with WT CARM1, but not in HEK293T CARM1 KO cells restored with R551K mutant CARM1 cell lysate.

Target description

~62 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 10% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_954592. UniProt accession no.: Q86X55. shipped in: dry ice. target post-translational modification: monomethylation (Arg551). Gene Information: human ... CARM1(10498). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.