ANTI-METTL14 CLONE 2G22 ZOOMAB RABBIT MONOCLONAL (C005B-261989)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2G22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Methyltransferase-like protein 14 (METTL14). It targets an epitope within 16 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Methyltransferase-like protein 14 (METTL14).

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected METTL14 in Raji cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected METTL14 in HeLa, A431, HUVEC, and NIH3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected METTL14 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-METTL14, clone 2G22 ZooMAb^®, Cat. No. ZRB1339, is a recombinant Rabbit monoclonal antibody that detects METTL14 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

N6-adenosine-methyltransferase non-catalytic subunit (UniProt: Q9HCE5; also known as Methyltransferase-like protein 14, hMETTL14) is encoded by the METTL14 (also known as KIAA1627) gene (Gene ID: 57721) in human. METTL14 forms a heterodimer with METTL3 to form a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates circadian clock, differentiation of embryonic stem cells, and cortical neurogenesis. This METTL14-METTL3 is an antiparallel heterodimer with active methyltransferase activity. In this heterodimer METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A) is one of the most abundant post-transcriptional, reversible modification of RNA in eukaryotic cells. It is a developmentally regulated RNA modification that is dynamically modified. It is an important physiological regulator involved in cell renewal and differentiation and may function in post-transcriptional fine-tuning of gene expression and may provide a faster means of maximizing gene expression. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Meyer, KD., et al. (2012). Cell. 149(7); 1635-1646).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.