ANTI-METTL7A CLONE 1O8 ZOOMAB RB MONO (C15-1670-982)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1O8 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human Methyltransferase-like protein 7A (METTL7A). It targets an epitope within 21 amino acids from the C-terminal region and detects both isoforms.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Methyltransferase-like protein 7A (METTL7A).

Application

Quality Control Testing

Evaluated by Western Blotting in HT-1376 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Methyltransferase-like protein 7A (METTL7A) in HT-1376 cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound METTL7A peptide with a KD of 1.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in human testis tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected METTL7A in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-METTL7A, clone 1O8 ZooMAb^®, Cat. No. ZRB1185, is a rabbit recombinant monoclonal antibody that targets METTL7A and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Methyltransferase-like protein 7A (UniProt: Q9H8H3 and computationally mapped F8VQX6, Q9H8H3, also known as AAM-B, METTL7A) is encoded by the METTL7A gene (Gene ID: 25840) in human. METTL7A is a ubiquitously expressed lipid droplets-associated protein that displays high expression in liver and thyroid tissues. It is synthesized with a signal peptide (aa 1-29) and is an integral membrane protein anchored into the endoplasmic reticulum (ER) membrane. It contains N and C-terminal domains, juxtamembrane region, and a putative central catalytic domain for methyltransferase activity. The N-terminal regions contains a hydrophobic sequence of 28 amino acids that is shown to be essential for its insertion into ER. It also helps recruit other cellular protein to lipid droplets for the formation of functional organelles. METTL7A is reported to be essential for the assembly and release stages of hepatitis C (HCV) life cycle but is not essential for the replication step of virus. Knockdown of METTL7A is shown to impair HCV propagation. METTL7A gene is reported to be downregulated in thyroid cancer. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, S., et al., (2017). Oncotarget. 8(21); 34652-34660; Park, EM., et al. (2015). PLoS One. 10(7); e0132839).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.