Anti-Microtubule-Associated Protein 2 (MAP2) Antibody (C15-1316-312)

General description

The microtubule-associated protein 2 (MAP2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). In neurons MAP2 occurs as three primary isoforms, the high molecular weight MAP2a, MAP2b, and the low molecular weight MAP2c, that result from alternative splicing of the MAP2 gene (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP2 together with MAP4 and tau proteins belong to the family of thermostable proteins associated with microtubules.

Specificity

Reactivity with other species has not been determined.

Specific for Microtubule-associated protein 2 (MAP2). The antibody recognizes all MAP2 isoforms (MAP2A, MAP2B, MAP2C and MAP2D). It shows greatest immunoreactivity with the HMW-MAP2 (MAP2A and MAP2B).

Immunogen

Purified Microtubule-associated protein from rat brain.

Application

Detect Microtubule-Associated Protein 2 (MAP2) using this Anti-Microtubule-Associated Protein 2 (MAP2) Antibody validated for use in ELISA, IC, IH, IH(P) & WB with more than 55 product citations.

Immunocytochemistry:
1:1,000 dilution of a previous lot was used on primary neurons.

Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.

Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).

ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Quality

Immunohistochemistry(paraffin) Analysis:
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum

Target description

Approx. 280 kDa doublet (MAP2a and MAP2b) and approx. 70 kDa doublet (MAP2c) on SDS-PAGE.

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain at -20ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeatedfreeze/thaw cycles.

Analysis Note

Control
Human brain tissue or human glioblastoma T98G cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: saturated ammonium sulfate (SAS) precipitated. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse, human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. NCBI accession no.: NM_001039538.1. UniProt accession no.: P11137. target post-translational modification: unmodified. Gene Information: human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595). Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.