Anti-Mitochondrial fission 1 protein (Fis1) Antibody (C15-1316-749)

General description

Mitochondrial fission 1 protein, hFIs1, is an overexpressed mammlian protein that is localized to the outer mitochondrial membrane of various cell types. It is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, resulting in the release of cytochrome c from the mitochondrion, and apopotosis. It is known to mediate peroxisomal fission and also plays a critical role in histone function by demethylating Lys-4 of histone H3.

Specificity

Other homologies: Rat and Mouse (83% sequence homology).

This antibody recognizes the cytoplasmic topological domain of Mitochondrial fission 1 protein.

Immunogen

Epitope: Cytoplasmic topological domain

KLH-conjugated-linear peptide corresponding to the cytoplasmic topological domain of human Mitochondrial fission 1 protein.

Application

Detect Mitochondrial fission 1 protein (Fis1) using this Anti-Mitochondrial fission 1 protein (Fis1) Antibody validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Mitochondrial fission 1 protein in 10 µg of Raji cell lysate.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_057152. UniProt accession no.: Q9Y3D6. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... FIS1(51024). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.