Anti-Mitofusin-1 Antibody (C15-1316-707)

General description

Along with Mitofusin-2 (Mfn2), Mitofusin-1 (Mfn1) is a known protein mediator of mitochondrial fusion. Mitochondrial fusion regulates mitochondrial morphogensis and supports complementation between mitochondrial genomes in mammalian cells. Two Mfn1 transcripts are detected at similar levels in a variety of human tissues and are dramatically elevated in the heart. Human Mfn1 is known to be localized in the mitochondria. It is reported that point mutations in the conserved G1 and G2 region of the Mfn1 GTPase domain result in decreased formation and fragmentation of the mitochondria, respectively.

Specificity

This antibody recognizes Mitofusin-1 at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic Domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Mitofusin-1.

Application

Detect Mitofusin-1 using this Anti-Mitofusin-1 Antibody validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Organelle & Cell Markers

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Mitofusin-1 in 10 µg HeLa cell lysate.

Target description

~86 kDa observed.
Three isoforms of Mitofusin-1 are produced by alternative splicing with molecular weights of ~42 kDa, ~71 kDa, and ~84 kDa Uncharacterized bands may appear at ~45 kDa, ~48 kDa, and ~55 kDa in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat, mouse. technique(s): western blot: suitable. NCBI accession no.: NP_284941. UniProt accession no.: Q8IWA4. target post-translational modification: unmodified. Gene Information: human ... MFN1(55669). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.