Anti-MIXL1 Antibody (C15-1317-853)

General description

MIXL1 is also known as Homeobox protein MIXL1, Homeodomain protein MIX (hMix), MIX1 homeobox-like protein 1, and Mix.1 homeobox-like protein. MIXL1 is a transcription factor with roles in axial mesendoderm morphogenesis, endoderm formation, blood cell differentiation, and morphogenesis of the heart and the gut during embryogenesis. MIXL1 has been shown to negatively regulate brachyury expression. MIXL1 is expressed mainly in progenitors and secondary lymph tissues, immature B- and T-lymphoid cells during normal hematopoiesis, and is also present in differentiating embryonic stem cells.

Specificity

Other homologies: Mouse and Rat (85% sequence homology).

This antibody recognizes the homeobox DNA binding domain of MIXL1.

Immunogen

Epitope: Homeobox DNA binding domain

KLH-conjugated linear peptide corresponding to the homeobox DNA binding domain of human MIXL1.

Application

Anti-MIXL1 Antibody is a highly specific rabbit polyclonal antibody, that targets Homeobox protein MIXL1 & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MIXL1 in H9 embryoid body cells. This protein was observed in H9 embryoid bodies (Lee, M. R., et al. (2010). Stem Cells. 28:1550-1559). MIXL1 was also localized in the nucleus of COS-7 and mouse ES cells (Mossman, A. K., et al. (2005). Stem Cells and Development. 14:656-663).

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MIXL1 in 10 µg of Jurkat cell lysate.

Target description

~36 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): primate (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_114150. UniProt accession no.: Q9H2W2. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... MIXL1(83881). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.