ANTI-MMP-3 ANTIBODY NT (C15-1315-993)

General description

MMP-3 (matrix metalloproteinase-3), also known as stromelysin-1, is a member of the matrix metalloproteinase (MMP) family of zinc endopeptidases. MMP’s are required for removal of extracellular matrix machinery during embryo development, morphogenesis and tissue remodeling. Endogenous tissue inhibitors of metalloproteinases (TIMPs) are responsible for regulating MMP-3. Any disruption of this system can cause diseases such as arthritis, metastasis, atherosclerosis and tumor growth.

Specificity

Other homologies: Mouse and rat (64% sequence homology).

This antibody recognizes MMP-3 at the N-terminus.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to human N-terminal MMP-3.

Application

Detect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected MMP-3 in colorectal carcinoma and ductal carcinoma tissues.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Quality

Evaluated by Western Blot in PMA-treated HL-60 concentrated media.

Western Blot Analysis: 0.1-1 µg/mL of this antibody detects MMP-3 in 10 µg of PMA-treated HL-60 concentrated media.

Target description

~58 kDa observed.
An uncharacterized band may be seen at ~78 kDa

Linkage

Replaces: AB812

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
PMA-treated HL-60 concentrated media

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rhesus macaque, human. species reactivity (predicted by homology): chimpanzee (100% sequence homology), rhesus monkey (100% sequence homology). packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_002413. UniProt accession no.: P08254. shipped in: ambient. storage temp.: 2-8°C. target post-translational modification: unmodified. Gene Information: human ... MMP3(4314). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.