ANTI-MOUSE CD25 (PC61.5) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 61.5 is a rat monoclonal antibody that detects Murine CD25.

Immunogen

Mouse CD25. The exact immunogen information is proprietary.

Application

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse splenocytes.

Flow Cytometry Analysis: Staining of one million BALB/c mouse splenocytes was performed with APC-conjugated anti-mouse CD4 and with 5 μL of a 1:1 mixture of Cat. No. CWA-1154, Anti-Mouse CD25 (PC61.5) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD25 expression was observed in part of CD4 positive population.

Tested Applications

Flow Cytometry Analysis: Staining of one million BALB/c mouse splenocytes was performed with PE-conjugated anti-mouse CD4 and with 5 μL of a 1:1 mixture of Cat. No. CWA-1154, Anti-Mouse CD25 (PC61.5) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD25 expression was observed in part of CD4 positive population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Interleukin-2 receptor subunit alpha (UniProt: P01590; also known as IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25) is encoded by the Il2ra (also known as Il2r) gene (Gene ID: 16184) in murine species. CD25 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 22-236), a transmembrane domain (aa 237-257), and a short cytoplasmic domain (aa 258-268). CD25 is mainly expressed on activated T -cells where it serves as a receptor for Interleukin-2 and is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity that suppresses the activation and expansion of autoreactive T-cells. The IL-2 receptor (IL-2R) is composed of three different subunit complexes, the - (CD25), β- (CD122) and -chains (CD132). By itself, CD25 is not sufficient to trigger the IL-2 signaling pathway because it is a low-affinity form of the IL-2 receptor (KD = 10 8 M) that is non-functional with respect to IL-2 internalization and signaling due to its short cytoplasmic tail. It is mainly involved in IL-2 binding thereby stabilizing a secondary binding site for the β-chain and recruiting -chain to the cell surface. (Ref.: Nihei, J., et al. (2014). Eur. J. Microbiol. Immunol. 4(2); 128-137; Ortega, G., et al. (1984). J. Immunol. 133(4); 1970-1975).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.