ANTI-MOUSE CD357 (DTA-1) COLORWHEEL DYE-READY MAB

General description

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone DTA-1 is a rat monoclonal antibody that detects CD357 (GITR). It targets an epitope within the extracellular domain.

Immunogen

Mouse CD25+ CD4+ T cells

Application

Quality Control Testing

Evaluated by Flow Cytometry in Mouse splenic CD4+ T cells.

Flow Cytometry Analysis: Staining of one million mouse splenic CD4+ T cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1116, Anti-Mouse CD357 (DTA-1) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1012 Rat IgG2b isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million mouse splenic CD4+ T cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1116, Anti-Mouse CD357 (DTA-1) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1012 Rat IgG2b isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Tumor necrosis factor receptor superfamily member 18 (UniProt: O35714; also known as Glucocorticoid-induced TNFR-related protein, CD357) is encoded by the Tnfrsf18 (also known as Gitr) gene (Gene ID: 21936) in murine species. CD357 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-153), a transmembrane domain (aa 154-174), and a cytoplasmic domain (aa 175-228). The mature form also contains three TNFR-Cys repeats (aa 28-61; 62-101; 102-142). CD357 is expressed in lymph node and peripheral blood leukocytes and weakly in spleen. Its expression is up regulated in peripheral mononuclear cells after antigen stimulation/lymphocyte activation. It is preferentially expressed in activated T lymphocytes and serves as a receptor for TNFSF18 (hGITRL It is involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. It is shown to be a crucial player in the differentiation and expansion of thymic Tregs (tTregs) and peripheral Tregs (pTregs). CD357 is suggested to be an attractive target for immunotherapy due to its capacity to promote effector T cell functions and hamper regulatory T cell suppression. (Ref.: Zappasodi, R., et al. (2019). Nat. Med. 25(5); 759-766; Ronchetti, S., et al. (2015). J Immunol Res. 2015; 171520).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.