ANTI-MOUSE CD40 (FGK45) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone FGK45 is a rat monoclonal antibody that detects murine CD40. It targets an epitope within the extracellular domain.

Immunogen

Recombinant Mouse CD40 Fusion Protein

Application

Quality Control Testing

Evaluated by Flow Cytometry in Mouse splenocytes.

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1136, Anti-Mouse CD40 (FGK45) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Tested Applications

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1136, Anti-Mouse CD40 (FGK45) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of APC-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Flow Cytometry in Mouse splenocytes.

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1136, Anti-Mouse CD40 (FGK45) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Tumor necrosis factor receptor superfamily member 5 (UniProt: P27512; also known as B-cell surface antigen CD40, Bp50, CD40L receptor, CD40) is encoded by the Cd40 (also known as Tnfrsf5) gene (Gene ID: 21939) in murine species. CD40 is a single-pass type I transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-193), a transmembrane domain (aa 194-205), and a cytoplasmic domain (aa 216-289). CD40 serves as a receptor for TNFSF5/CD40L. It is a costimulatory protein found on antigen-presenting cells and is required for their activation. Its expression has been observed in B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. It transduces TRAF-6 and MAP3K8-mediated signals that activate ERK in macrophages and B cells that leads to induction of immunoglobulin secretion. CD40 pathway is essential for the survival of many cell types including germinal center B cells, dendritic cells, and endothelial cells under normal and inflammatory conditions. Five isoforms of CD40 have been described that are produced by alternative splicing. (Ref.: Eliopoulos, AG., et al. (2003). EMBO J. 22(15); 3855-3864).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.