ANTI-MOUSE CD5 (53-7.3) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 53-7.3 is a rat monoclonal antibody that detects murine CD5. It binds to a monomorphic epitope on CD5.

Immunogen

Murine thymus/spleen cells

Application

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1032, Anti-Mouse CD5 (53-7.3) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

T-cell surface glycoprotein CD5 (UniProt: P13379; also known as Lymphocyte antigen 1, Ly-1, Lyt-1, CD5) is encoded by the Cd5 (also known as Ly-1) gene (Gene ID: 12507) in murine species. CD5 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-371), a transmembrane domain (aa 372-401), and a cytoplasmic domain (aa 402-494). In its extracellular domain it contains three scavenger receptor cysteine-rich (SRCR) domains. It is expressed on the surface of T cells bearing the β heterodimer T cell receptor (TCR) and a subset of murine B cells known as B-1a cells. It is either not present or is present at low density on the T cell subset. CD5 serves as a transmembrane receptor that regulates T cell proliferation, functions, and development. CD72 is recognized as one of the ligands for CD5. It is considered as an activation marker of T cells. Its expression is reported to increase according to the magnitude of the signal delivered by the TCR. It has been reported that the absence of CD5 in mice can enhance signaling and activation of double positive thymocytes induced by TCR stimulation, indicating that it acts as a negative regulator of TCR signaling in T cells. In mature, peripheral T cells, CD5 is reported to be present in lipid rafts of the T cell surface, where it promotes CD3 redistribution into rafts and upregulates ZAP-70 and LAT activation. (Ref.: Voisinne, G., et al. (2018). Front. Immunol. 9; 2900; Gary-Gouy, H., et al. (1997). J. Immunol. 159(8); 3739-3747).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.