ANTI-MOUSE CD86/B7-2 (GL-1) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone GL-1 is a rat monoclonal antibody that detects murine T-lymphocyte activation antigen CD86 (B7-2).

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Application

Quality Control Testing:

Evaluated by Flow Cytometry in 3-day LPS-stimulated BALB/c mouse splenocytes.

Staining of one million untreated BALB/c mouse splenocytes and LPS- treated (2 μg/mL for 3 days) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1055, Anti-Mouse CD86 (GL-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of a PE-conjugated rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

T-lymphocyte activation antigen CD86 (UniProt: P42082; also known as Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86) is encoded by the cd86 gene (Gene ID: 12524) in murine species. CD86 is a homodimeric, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-244), a transmembrane domain (aa 245-265), and a cytoplasmic domain (aa 266-309). It is constitutively expressed on interdigitating dendritic cells , Langerhans cells, memory B cells and germinal center B cells, and macrophages. It is rapidly upregulated on B cells following activation by cross-linking of the Ig receptor or by treatment with a variety of cytokines. Along with CD80 (B7-1) it provides costimulatory signals necessary for T cell activation and survival and displays about 25% homology with CD80. However, CD86 is more widely expressed and at higher levels than CD80. By binding to CD28 it provides costimulatory signals for T cell activation and survival and by binding to CTLA-4 (CD152) it plays a role in downregulating of T cell activity and immune regulation. Blocking the CD28-CD86 pathway is shown to induce cell death in myeloma cells and this is reported to be due to downregulation of integrins and interferon regulatory factor 4 (IRF4). (Ref.: Gavile, CM., et al. (2017). Blood Adv. 1(25); 2307-2319; Zheng, Y., et al. (2004). J. Immunol. 172(5); 2778-2784).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.