Anti-Mouse Collagen Type I Antibody (C15-1316-441)

General description

Collagen Type I extracted and purified from mouse skin. Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

Collagen alpha-1(I) chain (UniProt: P11087; also known as Alpha-1 type I collagen) is encoded by the Col1a1 (also known as Cola1) gene (Gene ID: 12842) in murine species. Collagen is the most abundant protein in the body and make up t 25% of the total protein content. It is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Collagen type I is a fibrillar type of collagen that is found in skin, tendons, ligaments, and bone and plays a major role in wound healing. Collagen type I is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-151) that are subsequently cleaved off to produce the mature form (aa 152-1207). It is expressed in almost all connective tissues and the predominant component of the interstitial membrane. It assembles into fibers that form the structural and mechanical scaffold of bone, skin, tendons, cornea, blood vessel walls and other connective tissues. Heterotrimers of two α1(I) and one α2(I) chains are shown to be the dominant isoform of type I collagen. However, homotrimers of three α1(I) chains have also been reported in fetal tissues, tumors, and in some fibrotic lesions. The homotrimeric isoform is shown to be more resistant to cleavage by collagenases. Type I collagen undergoes several post-translational modifications, some of these are formed during its synthesis to ensure mechanical competence of the fibrils and others formed as a function of aging and disease, such as cleavage and glycation that often results in reduced competence of the fibrils.

Specificity

Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

Recognizes mouse Collagen Type I.

Immunogen

Collagen Type I extracted and purified from mouse skin.

Application

Anti-Mouse Collagen Type 1 is a rabbit polyclonal antibody that detects mouse collagen type 1 and has tested for use in ELISA, Immunohistochemistry, Radioimmunoassay, and Western Blotting.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot Analysis:
1:500 dilution of a previous lot detected Collagen Type I on 10 μg of Mouse Liver lysate.

Immunohistochemistry:
1:40 dilution of a previous lot for immunofluorescent staining of frozen mouse skin and liver tissues.

RIA:
A 1:200 dilution of a previous lot was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA

Target description

MW of Collagen Type I precursor: 138,032 kDa

Linkage

Replaces: AB765

Physical form

Format: Purified

Protein A purified

The purified antibody is supplied in a buffer containing 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10 µg/ml of antibiotics and antimytotics

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Mouse skin and mouse liver tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NC_000077.7. UniProt accession no.: P11087. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... COL1A1(1277). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.