General description
Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria, and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells,,. Goat anti-mouse polyvalent immunoglobulins (G,A,M)-peroxidase antibody binds to mouse IgG, IgA and IgM.
Application
The detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Relative binding affinities for specific Mabs were determined by an inhibition ELISA using peroxidase-conjugated goat anti-mouse polyvalent antibody diluted 1:10,000 in PBST and incubated for 1 hour at 21-23°C.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: goat. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: affinity isolated antibody. antibody product type: secondary antibodies. clone: polyclonal. species reactivity: mouse. technique(s): direct ELISA: 1:10,000 using IgG, IgA, IgM. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317 - H412. Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313. Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41181529
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- A0412-1ML
- Temperature Control Device:
- Yes
