ANTI-MOUSE TCR ? (H57-597) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone H57-597 is an Armenian Hamster monoclonal antibody that detects murine TCR β. It targets an epitope within the constant region.

Immunogen

Affinity purified TCR from mouse DO-11.10 cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in EL4 cells.

Flow Cytometry Analysis: Staining of one million EL4 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1134, Anti-Mouse TCR β (H57-597) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye an equivalent amount of PE-conjugated Armenian Hamster IgG isotype control.

Tested Applications

Flow Cytometry Analysis: Staining of one million EL4 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1134, Anti-Mouse TCR β (H57-597) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Armenian Hamster IgG isotype control.

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse CD4+ T cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1134, Anti-Mouse TCR β (H57-597) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount APC-conjugated Armenian Hamster IgG isotype control.

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse CD4+ T cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1134, Anti-Mouse TCR β (H57-597) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Armenian Hamster IgG isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Flow Cytometry in EL4 cells.

Flow Cytometry Analysis: Staining of one million EL4 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1134, Anti-Mouse TCR β (H57-597) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye an equivalent amount of PE-conjugated Armenian Hamster IgG isotype control.

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

T cell receptors recognize foreign antigens that have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one and one β chain or one and one chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order , , β, and . If both and rearrangements produce functional chains, the cell expresses and . If not, the cell proceeds to rearrange the β and loci. This region represents the germline organization of the T cell receptor β locus. The β locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the β chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the β locus are known to be incapable of encoding a protein and are considered pseudogenes. The β locus also includes several trypsinogen genes. Within the TCR β chain, the complementarity-determining region (CDR) 1 and 2 loops of the TCR contact the MHC -helices while the hypervariable CDR3 regions interact mainly with the peptide. In both TCR and TCR β chains, CDR3 loops have the highest sequence diversity and are the principal determinants of receptor binding specificity. Clone H57-597 specifically reacts with surface receptors on all β TCR-bearing cells and does not react with receptors on + T cells. It is reported to react with the constant region of TCR β. (Ref.: Springer, I., et al. (2021). Front. Immunol. 12; 664514; Gregoire, C., et al. (1991). Proc. Natl. Acad. Sci USA. 88(18); 8077-8981; Kubo, RT., et al. (1989). J. Immunol. 142(8); 2736-2742).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.