ANTI-ROOTLETIN (C15-1317-826)

General description

Prostaglandin E synthase (UniProt: O14684; also known as EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein) is encoded by the PTGES gene (Gene ID: 9536) in human. mPGES -1 is a multi-pass membrane protein that is integral membrane, inducible and glutathione-dependent terminal synthase in the prostanoid biosynthesis pathway and acts downstream cyclooxygenases (COX) catalyzing the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Functionally, mPGES-1 is coupled to COX-2 and the induction of these two enzymes by pro-inflammatory cytokines, growth factors or endotoxin leads to a surge of PGE2 synthesis by inflammatory cells. It is strongly expressed with COX-2 in synovial fibroblasts and macrophages. In cases of osteoarthritis, mPGES-1 and COX-2 are strongly expressed in the chondrocytes and the synovial lining cells. mPGES-1 also play a role in the pathogenesis of ischemic stroke and many neurodegenerative diseases. In Alzheimer disease brains higher expression of both COX-2 and mPGES-1 is observed. Higher expression of mPGES has also been reported in several cancers and this higher expression has been correlated with vascular invasion and worse prognosis in colorectal cancer. A549 and DU145 xenografts lacking mPGES-1 display significantly reduced growth rates. (Ref.: Korotkova, M., and Jakobsson, PJ (2014). Basic Clin. Pharmacol. Toxicol. 114, 64-69).

Specificity

This polyclonal antibody specifically detects mPGES-1 in human.

Immunogen

His-tagged full-length recombinant human microsomal prostaglandin E synthase.

Application

Detect Prostaglandin E synthase using this rabbit polyclonal Anti-mPTGES-1, Cat. No. ABS2192 that has been tested in Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence, and Western Blotting.

Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected mPTGES-1 in human tonsil and human placenta tissue.

Western Blotting Analysis: A representative lot detected mPTGES-1 in human chondrocytes (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9) and wild-type mixed glial cultures (Straccia, M., et. al. (2013). Glia. 61(10):1607-19).

Immunofluorescence Analysis: A representative lot detected mPTGES-1 in Wild-type mixed glial cultures (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

Western Blotting Analysis: A representative lot detected mPTGES-1 in PNE inhibitedmPGES-1proteinexpressioninIL-1 -stimulated SK-N-SH cells (Olajide, O.A., et. al. (2014). J Ethnopharmacol. 152(2):377-83).

Immunohistochemistry Analysis: A representative lot detected mPTGES-1 in fresh frozen brain tissue (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

Quality

Evaluated by Western Blotting in recombinant microsomal Prostaglandin E synthase 1 protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mPTGES-1 in recombinant microsomal Prostaglandin E synthase 1 protein.

Target description

~17 kDa observed; 17.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit polyclonal antiserum without azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: unpurified. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. species reactivity (predicted by homology): rat (based on high sequence homology). technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. isotype: IgG. NCBI accession no.: NP_004869. UniProt accession no.: O14684. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... PTGES(9536). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.