Anti-MTNR1B (C15-1317-347)

General description

Melatonin receptor type 1B (UniProt: P49286; also known as Mel-1B-R; Mel1b receptor) is encoded by the gene MTNR1B gene (Gene ID 4544) in human. Mel-1B-R is a high affinity receptor for melatonin that belongs to the G-protein coupled receptor 1 family. Mel-1B mediates the reproductive and circadian actions of melatonin. Melatonin is shown to be involved in body mass regulation in mammals and this effect is suggest to be mediated by a direct action of melatonin on Mel-1B receptors on brown adipocytes. Melatonin also acts as a chemopreventive agent and its levels inversely correlate with the risk of developing cancer. In this regard melatonin induces p38-dependent phosphorylation of both p53 and histone H2AX. Activation of the p53-dependent DNA damage response by melatonin is mediated by both Mel-1A and Mel-1B receptors. The absence of either receptor impairs melatonin′s ability to reduce both cell proliferation and clonogenic potential of cancer cells. (Brydon, L. et al. (2001). Endocrinology. 142(10):4264-4271; Santoro, R., et al. (2013). Carcinogenesis. 34(5):1051-1061).

Specificity

This polyclonal antibody detects melatonin receptor type 1B in human hippocampus membrane.

Immunogen

A KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human melatonin receptor type 1B.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MTNR1B in human retina and human melanoma tissue.

Research Category
Stem Cell Research

This rabbit polyclonal Anti-MTNR1B, Cat. No. ABN1370 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Melatonin receptor type 1B.

Quality

Evaluated by Western Blotting in human hippocampus membrane.

Western Blotting Analysis: 2 mg/mL of this antibody detected MTNR1B in 10 mg of human hippocampus membrane.

Target description

~38 kDa observed; 40.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_005950. UniProt accession no.: P49286. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... MTNR1B(4544). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.