Anti-MTNR1B (C15-1318-020)

General description

Melatonin receptor type 1B (UniProt: P49286; also known as Mel-1B-R, Mel1b receptor) is encoded by the MTNR1B gene (Gene ID: 4544) in human. Mel-1B-R is a member of the G-protein coupled 1 family that is predominantly expressed in retina and to lesser extent in brain tissue. . Mel-1B-R activity is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Mel-1B-R mediates the reproductive and circadian actions of melatonin. Melatonin is shown to be involved in body mass regulation in mammals and this effect is suggest to be mediated by a direct action of melatonin on Mel-1B receptors on brown adipocytes. Melatonin is reported to act as a chemopreventive agent and its levels inversely correlate with the risk of developing cancer. In this regard melatonin induces p38-dependent phosphorylation of both p53 and histone H2AX. Activation of the p53-dependent DNA damage response by melatonin is mediated by both Mel-1A and Mel-1B receptors. The absence of either receptor impairs melatonin′s ability to reduce both cell proliferation and clonogenic potential of cancer cells. A variant in MTNR1B gene is reported to worsen the deleterious effect of melatonin on glucose tolerance in humans. (Ref.: Brydon, L. et al. (2001). Endocrinology. 142(10):4264-4271; Santoro, R., et al. (2013). Carcinogenesis. 34(5):1051-1061).

Specificity

This rabbit polyclonal antibody detects Melatonin receptor type 1B in human.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Melatonin receptor type 1B.

Application

Anti-MTNR1B Antibody, Cat. No. ABT1890, is a rabbit polyclonal antibody that detects MTNR1B and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MTNR1B in human retina tissue.

Research Category
Cell Structure

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MTNR1B in 10 µg of HepG2 cell lysate.

Target description

40.19 kDa calculated.

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_005950.1. UniProt accession no.: P49286. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... MTNR1B(4544).