ANTI-MUCIN-16 (CA-125) CLONE 5E11 (C005B-451723)

General description

Mucin-16 (UniProt: Q8WXI7; also known as MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125) is encoded by the MUC16 (also known as CA125) gene (Gene ID: 94025) in human. Mucin-16 is a heavily O-glycosylated protein that can be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus, which induces proteolytic cleavage and liberation of extracellular domain. I provides protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Mucin-16 is composed of three domains: a Serine, Threonine-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane domain with a short cytoplasmic tail. Its expression is up-regulated in ovarian cancer cells and may serve as a marker for ovarian cancer. Mucin-16 is shown to binds to mesothelin (MSLN) and this binding mediates heterotypic cell adhesion, which may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium.

Specificity

Clone 5E11 detects Mucin-16 in human ovarian cancer tissue. It targets an epitope within 264 amino acids from the C-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 264 amino acids from the C-terminal half of human Mucin-16.

Application

Anti-Mucin-16 (CA-125), clone 5E11, Cat. No. MABC1608, is a mouse monoclonal antibody that detects Mucin-16 and it has been tested for use in Immunohistochemistry (Paraffin), Proximity Ligation Assay, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (Courtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).

Proximity Ligation Assay Analysis: A representative lot detected Mucin-16 in Proximity Ligation Assay applications (Ricardo, S., et. al. (2016). Virchows Arch. 468(6):715-22; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).

Immunohistochemistry Analysis: A representative lot detected Mucin-16 in Immunohistochemistry applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).

Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (Courtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).

Western Blotting Analysis: A representative lot detected Mucin-16 in Western Blotting applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82).

Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry in human ovarian cancer and human uterine tissues.

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution of this antibody detected Mucin-16 in human ovarian cancer and human uterine tissues.

Target description

1519.17 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.