General description
In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, cellular differentiation, nuclear signaling and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3′ UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.
Immunogen
GST-tagged recombinant protein corresponding to human Musashi-1.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Neural Stem Cells
Use Anti-Musashi-1 Antibody, clone 7B11.1 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Musashi-1 also known as RNA-binding protein Musashi homolog 1.
Western Blot Analysis: A representative lot detected Musashi-1 in HEK293, NIH/3T3, and PC-12 cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Musashi-1 in mouse cerebellum, normal mouse brain, and normal human pons tissue.
Quality
Evaluated by Western Blot in mouse E17 spinal cord tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Musashi-1 in 10 µg of mouse E17 spinal cord tissue lysate.
Target description
~39 kDa observed
Linkage
Replaces: 03-114; 04-1041
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse E17 spinal cord tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51173210
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABE268
- Temperature Control Device:
- Yes
