ANTI-MYELIN ASSOCIATED GLYCOPROTEIN ANTIBODY CLONE 513

General description

Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.

Specificity

Detects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies.

Immunogen

L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.

Application

Detect Myelin Associated Glycoprotein using this Anti-Myelin Associated Glycoprotein Antibody, clone 513 validated for use in IC, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins

Western Blot:
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}

Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by immunohistochemistry on rat hippocampus.

Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.

Target description

100 kDa

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat hippocampus tissue, rat brain lysate, PC12 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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