ANTI-N-WASP ANTIBODY PHOSPHO-SPECIFIC [SER484/SER485] (C005B-239776)

General description

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.

Specificity

The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

Immunogen

Epitope: phosphoserine484/phosphserine485

Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.

Application

Detect N-WASP using this Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485] validated for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Western blot: 1:1000 ELISA: 1:2000

Physical form

This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Storage and Stability

Maintain at 20°C for up to one year from date of receipt. Do not aliquot.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NM_003941.2. UniProt accession no.: O00401. shipped in: wet ice. target post-translational modification: phosphorylation (pSer484/pSer485). Gene Information: human ... WASL(8976). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.

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