ANTI-N-WASP ANTIBODY PHOSPHO-SPECIFIC [TYR256] (C005B-239777)

General description

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

Specificity

The antibody detects a 65 kDa protein corresponding to the molecular weight of phosphorylated N-WASP on SDS-PAGE Western blots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine.

Immunogen

Epitope: phosphotyrosine 256

Phospho-N-WASP (Tyr256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.

Application

Anti-N-WASP Antibody, phospho-specific [Tyr256] detects level of N-WASP & has been published & validated for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Western blot: 1:10,000 ELISA: 1:20,000

Physical form

This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr256) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Storage and Stability

Maintain at 20°C for up to one year from date of receipt. Do not aliquot.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NM_003941.2. UniProt accession no.: O00401. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... WASL(8976). Storage Class Code: 10 - Combustible liquids.

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