Specificity
Specific for the α-1 subunit isoform.
Immunogen
Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Application
Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Quality
Immunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40μg of rat brain microsomal preparation.
Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.
Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).
Target description
113 kDa
Physical form
25µg Alexa Fluor™ 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween
Protein G Purified
Storage and Stability
Do Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.
Analysis Note
Control
Included Negative Control: Catalog # 16-239, Alexa Fluor™ 555-conjugated Normal Mouse IgG.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51172838
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 05-369X-555
- Temperature Control Device:
- Yes