Anti-Nanog Antibody (C15-1316-480)

General description

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

Specificity

Recognizes Nanog, human.

Immunogen

Synthetic peptide from human Nanog.

Application

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.

Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.

Target description

39 kDa (MW 34 kDa)

Physical form

Rabbit Serum. Liquid.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Whole cell lysate from mouse embryonic stem cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. manufacturer/tradename: Chemicon^®. . technique(s): flow cytometry: suitable, western blot: suitable. input: sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s). NCBI accession no.: NM_024865.2. UniProt accession no.: Q9H9S0. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NANOG(79923)
mouse ... Nanog(71950). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.