Anti-NANOS3 Antibody (C15-1316-815)

General description

Nanos homolog 3 (Nanos3), also known as NOS-3 or NOS3, belongs to the Nanos family. Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. Nanos3 has been shown to play an important role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Nanos3 has also been shown to be essential in the early stage embryo protecting the migrating primordial germ cells (PGCs) from apoptosis. Additionally, it is implied to maintain the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways and to affect cell proliferation by repressing translation of specific mRNAs. Nanos3 is expressed primarily in ovary, testis and brain. In the ovaries, Nanos3 is expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, Nonos3 is expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.

Specificity

This antibody recognizes the C-terminus of NANOS3.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human NANOS3.

Application

Detect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS3 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NANOS3 in human brain cells.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS3 in 15 µg of human brain tissue lysate.

Target description

~21 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~19 kDa and ~21 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. UniProt accession no.: P60323. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NANOS3(342977). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.