Anti-NDRG1 Mouse mAb (2D7) (C15-1319-093)

General description

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.

Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.

This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.

Immunogen

Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (0.7 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human endometrium tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: 2D7, monoclonal. form: liquid. does not contain: preservative. species reactivity: human. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG2a. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... NDRG1(10397). Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.