Anti-NDST4 antibody produced in rabbit

General description

NDST4 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4) is the fourth member of NDST family, and shows high sequence homology to the other three members namely, NDST1, NDST2 and NDST3. It is made of 872 amino acids. This gene is localized to human chromosome 4q26, and alternative splicing gives rise to two isoforms of this protein. This protein is majorly expressed in developing embryos, and in adult brain. It is a type II transmembrane protein with 22 amino acids in the transmembrane region, and 12-13 amino acids in the cytoplasmic domain. It also shares two PAPS (3′-phosphoadenosine 5′-phosphosulfate) binding motifs, with other sulfotransferases.

Immunogen

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

The functionality of NDST4 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4) is not yet characterized. It has higher sulfotransferase activity and lower deacetylase activity. It catalyzes the synthesis of heparan sulfate (HS) on existing proteins to form heparan sulfate proteoglycans (HSPGs). This gene is down-regulated in colorectal cancer (CRC), and is associated with advanced cancer phenotype. It is associated with invasiveness and metastasis of CRC, which might be an outcome of alterations in the HS chains of certain HSPGs. Therefore, NDST4 might have potential as a marker to predict the prognosis of CRC. This protein might be responsible for the addition of sulfate groups to glucosaminyl residues, which were deacetylated and left unaltered by other NDST members. Genome-wide association studies show that SNP near this gene is associated with maximum number of drinks (MaxDrinks), which is related to alcohol dependence.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST71993.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.