Anti-Nestin, Human Antibody (C15-1316-827)

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Specificity

This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.

Immunogen

GST-tagged recombinant protein corresponding to human Nestin.

Application

Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.

Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.

Quality

Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.

Target description

The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments

Linkage

Replaces: AB5922

Analysis Note

Control
U251 glioblastoma cells.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. should not react with : rat, mouse. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_006608. UniProt accession no.: P48681. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NES(10763). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.