ANTI-NEUROFIBROMIN NF-1 CLONE NF1-A (C005B-449506)

General description

P2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 receptor is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Most of its transmembrane domains appear to be tilted and/or kinked. Binding of agonists to P2Y12 receptor promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. It serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is highly expressed in platelets and lower level of expression levels is observed in the brain, lung, and adrenal glands. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).

Specificity

Clone 10B5.3.27 is a mouse monoclonal antibody that detects P2Y purinoreceptor 12. It targets an epitope within 40 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

GST-tagged recombinant fragment corresponding to 40 amino acids from the C-terminal, cytoplasmic domain of human P2Y purinoreceptor 12.

Application

Quality Control Testing

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2RY12 in human brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected P2RY12 in U251 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected P2RY12 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-P2RY12, clone 10B5.3.27, Cat. No. MABN2593, is a mouse monoclonal antibody that detects P2Y purinoceptor 12 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.