Anti-Neurofilament L Antibody (C15-1316-535)

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10 nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Specificity

Recognizes Neurofilament-Light (NF-L)

Immunogen

Purified porcine NF-L

Application

Detect Neurofilament L using this Anti-Neurofilament L Antibody validated for use in IC, IH, IH(P) & WB.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Western Blot Analysis:
1:1000 dilution of a previous lot detected NEUROFILAMENT L on 10 μg of mouse brain lysates.

Immunocytochemistry:
A 1:200-1:500 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.

Quality

Immunohistochemistry(paraffin) Analysis:
Neurofilament-L (cat. # AB9568) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:800, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) around a Purkinje cells.
Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Human Cerebellum

Target description

68-70 kDa

Linkage

Replaces: 04-1112

Physical form

Rabbit serum. Contains no preservative.

Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, pig, human, bovine, feline, canine, mouse. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. NCBI accession no.: NM_006158.2. UniProt accession no.: P07196. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... NEFL(4747). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.