Anti-Neurogenin-2 Antibody (C15-1316-326)

General description

Neurogenin 2 (NGN2) is a helix-loop-helix class of transcription factor. Transcription factors with bHLH motifs modulate critical events in the development of the mammalian neocortex. The transition from proliferation to neurogenesis involves a coordinate increase in the activity of proneural bHLH factors, including Neurogenin 2 and a decrease in the activity of Hes and Id factors. bHLH factors have key roles in corticogenesis, affecting the timing of differentiation and the specification of cell fate. Neurog2 controls neurogenesis in the embryonic cerebral cortex directly induces the expression of the small GTP-binding protein Rnd2 in newly generated mouse cortical neurons before they initiate migration.

Specificity

Recognizes Neurogenin 2 (MATH4a), a Helix-loop-helix class of transcription factor.

Immunogen

Synthetic peptide, amino acids 85-97 of mouse Neurogenin 2.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

This Anti-Neurogenin-2 Antibody is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Neurogenin-2.

Western blot: 1-10 μg/mL using ECL.
Immunocytochemistry: Cat. # AB5682 detected Neurogenin-2 in PC12 cells at 1:500 dilution. It was also detected in paraformaldehyde-fixed differentiated human neural stem cells.
ELISA: 0.5-1 μg/mL using 1 mg/mL control peptide (Catalog Number AG352) per well.
Immunohistochemistry on paraffin sections has been reported by Lamolet, B. et al. (2004)
Optimal working dilutions must be determined by the end user.

Target description

~28 kDa

Linkage

Replaces: AB9620

Physical form

Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Storage and Stability

Stable for 1 year at -70ºC from date of receipt.

Analysis Note

Control
Human brain Lysate.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse, human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NM_024019.2. UniProt accession no.: Q9H2A3. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... NEUROG2(63973). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.