General description
Neurogenins are a family of bHLH transcription factors involved in specifying neuronal differentiation. They are related to Drosophila atonal. The neurogenins (ngns) make up one of these atonal-related gene families. In neural crest cells, the atonal-related neurogenin family is particularly important for the sensory lineage (Neurogenins Are Essential for the Formation of dorsal root ganglia), while the achaete-scute homologue ash1 (Mash1) is important for aspects of autonomic neurogenesis (mash1 is essential for noradrenergic differentiation). Neurogenin-3 also promotes pancreatic development.
Specificity
This antibody recognizes Neurogenin-3 at the N-terminus.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse Neurogenin-3.
Application
Detect Neurogenin-3 (rabbit) using this Anti-Neurogenin-3 (rabbit) Antibody validated for use in WB, IH.
Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Neurogenin-3 in mouse embryo E12.5+ tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Quality
Evaluated by Western Blot in mNgn3 transfected and wildtype rabbit reticulocyte lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neurogenin-3 on 10 µg of mNgn3 transfected rabbit reticulocyte lysates.
Target description
32 kDa
Physical form
Unpurified
Unpurified rabbit polyclonal serum containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
mNgn3 transfected and wildtype rabbit reticulocyte lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. technique(s): immunohistochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_033849. UniProt accession no.: P70661. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Neurog3(11925). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 51201516
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB10535