Anti-Neuropilin-2b (C15-1317-499)

General description

Neuropilin-2 (UniProt: O60462; also known as Vascular endothelial cell growth factor 165 receptor 2) is encoded by the NRP2 (also known as VEGF165R2) gene (Gene ID: 8828) in human. Neuropilin-2 is a single-pass type I membrane protein that serves as a high affinity receptor for VEGF165 and VEGF145, semaphorins 3C, 3F, and the PLGF-2 isoform of PGF. It is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved in the mature form. Neuropilin-2 has an extracellular domain (aa 21-864), a transmembrane domain (aa 865-889), and cytoplasmic tail (aa 890-931). It contains two CUB domains (aa 28-142 and 149-267) that mediate its binding to semaphorin. It also has two F5/8 type -C domains that are responsible for heparin and VEGF binding. Six different isoforms of Neuropilin-2 have been described that are produced by alternative splicing. In non small cell lung cancer (NSCLC) cell lines, TGF-beta signaling is reported to preferentially increase the abundance of NRP2b. Although it has been shown that the presence of NRP2b inhibits the proliferation of cultured cells and diminishes primary tumor growth, it also enhances cellular migration, invasion into Matrigel, and tumorsphere formation in cultured cells in response to TGF-beta. In addition, it is shown to promote metastasis in xenograft murine models. In lung cancer cell lines, knocking down of NRP2 is shown to impair both baseline and TGF-beta-induced migration and invasion. (Ref.: Gemmill, RM., et al., (2017). Sci. Signal. 10, eaag0528).

Specificity

This rabbit polyclonal antibody detects Neuropilin-2b in human and murine cells.

Immunogen

Epitope: C-terminus

Mixture of two KLH-conjugated peptides corresponding to 17 and 16 amino acids from the C-terminal region of human Neuropilin-2b.

Application

Anti-Neuropilin-2b, Cat. No. ABN2262, is a highly specific rabbit polyclonal antibody that targets Neuropilin-2b (NRP2b) and has been tested for use in Immunocytochemistry,Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Neuropilin-2b in Immunoprecipitation applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

Immunocytochemistry Analysis: A representative lot detected Neuropilin-2b in Immunocytochemistry applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

Western Blotting Analysis: A representative lot detected Neuropilin-2b in Western Blotting applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

Western Blotting Analysis: A 1:250 dilution from a representative lot detected Neuropilin-2b in HEK293 transfected with NRP2b (Courtesy of Dr. Robert Gemmill @ Medical University of South Carolina).

Immunohistochemistry Analysis: A representative lot detected Neuropilin-2b in Immunohistochemistry applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neuropilin-2b in NIH3T3 cell lysate.

Target description

~115 kDa observed. 104.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. packaging: antibody small pack of 25 . μ. g. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_061004, NP_957719. UniProt accession no.: O60462. target post-translational modification: unmodified. Gene Information: human ... NRP2(8828). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.