Specificity
NFkappaB p65, phosphoSer276. By Western blot the antibody shows minimal reactivity with non-phosphorylated p65 and by ELISA is shows minimal reactivity with the non-phosphorylated form of the immunogen peptide.
Immunogen
Epitope: phospho-specific (Ser276)
Synthetic peptide from human NFkappaB p65, phosphoSer276.
Application
Anti-NFκB p65 Antibody, phospho-specific (Ser276) is a high quality Rabbit Polyclonal Antibody for the detection of NFκB p65 & has been validated in ELISA & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blot: 1:1,000. A 65 kDa band is observed against human p65. HeLa cells of TNF inducible KBM-5 cells can be used as a positive control.
ELISA: 1:10,000-1:30,000
Optimal working dilutions must be determined by the end user.
Physical form
Rabbit serum. Liquid containing 0.1% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NM_021975.2. UniProt accession no.: Q04206. shipped in: wet ice. target post-translational modification: phosphorylation (pSer276). Gene Information: human ... NFKB1(4790). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 51202414
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB3375
