ANTI-NITROTYROSINE ANTIBODY

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.

Specificity

Recognizes proteins which are nitrated.

Immunogen

Nitrated KLH

Application

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

This Anti-Nitrotyrosine Antibody is published in more than 80 citations and validated for use in Immunocytochemistry (IC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB) for the detection of Nitrotyrosine.

Quality

routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)

Target description

Predicted MW for nitrated-BSA: 85 kDa

Physical form

Format: Purified

ImmunoAffinity Purified

Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Immunohistochemistry: Human Alzheimer disease brain sections; mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: Nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.