General description
Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.
Immunogen
peroxynitrite-treated KLH
Application
Immunoblotting (2 µg/ml)
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
Reconstitution
Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Mouse macrophages treated with peroxynitrite
Other Notes
Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: CC22.8C7.3, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity (predicted by homology): all. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG2b. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... NOS1(4842). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51286803
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 487923-50UG