ANTI-NKP46 (CD335) CLONE 461-G1 (C15-1887-138)

General description

Natural cytotoxicity triggering receptor 1 (UniProt: O76036; also known as Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, hNKp46, CD335) is encoded by the NCR1 (also known as LY94) gene (Gene ID: 9437) in human. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis and elimination of virus-infected cells. It is considered as a primary activating receptor mediating NK cell antiviral function. It is involved in the functional recognition of viral HA and hemagglutinin-neuraminidases. NKp46 is shown to be predominantly expressed in the nasal lavage of patients infected with respiratory viruses, but not in normal healthy subjects. Following the initiation of an NK-target cell interaction, NKp46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. Human NK-p46 is synthesized with a signal peptide of 21 amino acids, which is subsequently cleaved off. The mature form has an extracellular domain (aa 22-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-304). It also has two Ig-like domains (aa 34-118 and 129-211) termed as D1 and D2. Five different isoforms of NKp46 have been described that are produced by alternative splicing. (Ref.: Shemer-Avni, Y., et al., (2017). Front. Immunol. 8 (Article 161); 1-10).

Specificity

Clone 461-G1 specifically recognizes whole human NKp46 and NKp46-D1, but does not recognize NKp46-D2. It targets an epitope with in the first 100 amino acids from the extracellular domain.

Immunogen

Human IgG1 Fc-fused to a recombinant fragment corresponding to the first 100 amino acids from the extracellular domain of human NKp46.

Application

Anti-NKp46 (CD335), clone 461-G1, Cat. No. MABF2059, is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 1 and has been tested for use in ELISA and Flow Cytometry.

Flow Cytometry Analysis: 4 µg/mL from a representative lot detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel).

ELISA Analysis: A representative lot detected NKp46 (CD335) in ELISA applications (Yossef, R., et. al. (2015). PLoS One. 10(2):e0118936).

Research Category
Inflammation & Immunology

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Target description

34.48 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.