ANTI-NKP46 (CD335) CLONE WEN23 (C15-1887-226)

General description

Natural cytotoxicity triggering receptor 1 (UniProt: Q9Z0H5; also known as Activating receptor 1, rAR-1, Lymphocyte antigen 94 homolog, NK receptor KILR-1, NKACTR, Natural killer cell p46-related protein, NK-p46, NKp46, CD335) is encoded by the Ncr1 (also known as Ar1, Kilr1, Ly94) gene (Gene ID: 117547) in rat. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a NK-p46 is an activating NK cell receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NK-p46 also regulates graft-versus-host disease and allergic response. Following the initiation of an NK-target cell interaction, NK-p46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. NK-p46 has an extracellular domain (aa 17-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-325). It also has two Ig-like domains (aa 42-100 and 137-192). It is synthesized with a signal peptide of 16 amino acids, which is subsequently cleaved off. NK-p46 is expressed by all natural killer (NK) cells, but not by T cells, B cells, granulocytes, monocytes, dendritic cells, or macrophages. It is weakly expressed in spleen, heart, and lung tissue. The main NK cell population of the lymph node expresses low levels of NK-p46 in resting cells, however, its expression is elevated by the presence of interleukin-2. (Ref.: Hadad, U et al. (2015). Front. Immunol 60 (Article 495); 1-11).

Specificity

Clone WEN23 detects natural cytotoxicity receptor 1 (NCR1, NK-p46) in rat. It targets an epitope in the ectodomain regioin.

Immunogen

Recombinant rat NKp46 ectodomain coupled to the Fc portion of mouse IgG2b.

Application

Anti-NKp46 (CD335), clone WEN23, Cat. No. MABF1970 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 1 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Affects Function and Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: A representative lot detected NKp46 (CD335) in Western Blotting applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

Affects Function Analysis: A representative lot of anti-NKp46 (CD335) affected funtion of NKp46 (CD335). (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

Immunoprecipitation Analysis: A representative lot detected NKp46 (CD335) in Immunoprecipitation applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

Immunohistochemistry Analysis: A representative lot detected NKp46 (CD335) in Immunohistochemistry applications (Smelt, M.J., et. al. (2014). Cell Transplant. 23(11):1381-94).

Quality

Evaluated by Flow Cytometry in HEK293 cells transfected with rat NKp46.

Flow Cytometry Analysis: 1 µg of this antibody detected NKp46 (CD335) in HEK293 cells transfected with rat NKp46.

Target description

37.18 kDa calculated.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.