ANTI-NKR-P1G CLONE ADA3 (C005B-450663)

General description

CKLF-like MARVEL transmembrane domain-containing protein 6 (UniProt: Q9NX76; also known as Chemokine-like factor superfamily member 6, CMTM6) is encoded by the CMTM6 gene (Gene ID: 54918) in human. CMTM6 is a ubiquitously expressed, multi-pass membrane protein of the family of MARVEL domain-containing proteins with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. Its MARVEL domain is localized in amino acids 33-160. It serves as a master regulator of recycling and plasma membrane expression of PD-L1. It binds to PD-L1 and maintains its cell surface expression. Although it is not required for PD-L1 maturation, but it co-localizes with PD-L1 at the plasma membrane and in recycling endosomes, where it prevents PD-L1 from being targeted for lysosome-mediated degradation by preventing its STUB-1 mediated ubiquitination. It also stabilizes PD-L1 expression on antigen presenting cells and potentiates inhibitory signaling by PD-1 (CD279), ultimately triggering T-cell anergy. It enhances the ability of PD-L1-expressing tumor cells to inhibit T cell activity. (Ref.: Burr, ML., et al. (2017). Nature. 549(7670); 101-105; Mezzadra, R., et al. (2017). Nature. 549(7670); 106-110).

Specificity

Clone RCT6 is a mouse monoclonal antibody that detects human CMTM6. It targets an epitope within the C-terminal region.

Immunogen

A linear peptide corresponding to a sequence from the C-terminal region of human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6).

Application

Quality Control Testing

Evaluated by Western blotting in lysates from 8505c cells overexpressing CMTM6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CMTM6 in lysates from 8505C cells overexpressing CMTM6, but not in knockout or untransfected cells.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CMTM6 in Immunohistochemistry applications (Mezzadra, R., et al. (2017). Nature. 549(7670):106-110).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CMTM6, clone RCT6, Cat. No. MABF1988, is a mouse monoclonal antibody that detects CMTM6 and is tested for use in Immunohistochemistry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.