Anti-NLRP12 Antibody (C15-1317-303)

General description

NLRP12 (NACHT, LRR and PYD domains-containing protein 12) is also known as Monarch-1 or PYRIN-containing APAF1-like protein 7 (PYPAF7). NLRP12 is thought to mediate activation of CASP1 (via ASC) and NF-kappa-B (via IKK). NLRP12 has been detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes, and is regulated by nitric oxide. NLRP12 defects can cause familial cold autoinflammatory syndrome type 2 (FCAS2).

Specificity

Other homologies: Mouse (91% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human NLRP12.

Application

Anti-NLRP12 Antibody is a highly specific rabbit polyclonal antibody, that targets NLRP & has been tested in western blotting & Flow Cytometry.

Flow Cytometry Analysis: 0.5 µg from a representative lot detected NLRP12 in rat basophil leukemia (RBL).

Research Category
Signaling

Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blotting in rat basophil leukemia (RBL) cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NLRP12 in 10 µg of rat basophil leukemia (RBL) cell lysate.

Target description

~110 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing this antibody recognizes isoforms 1 through 4 at ~120 kDa, ~114 kDa, ~107 kDa, and ~102 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat basophil leukemia (RBL) cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. species reactivity (predicted by homology): human (immunogen homology), bovine (based on 100% sequence homology). technique(s): flow cytometry: suitable, western blot: suitable. NCBI accession no.: NP_150639. UniProt accession no.: P59046. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NLRP12(91662). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.