ANTI-NLRP3 ANTIBODY (C15-1317-302)

General description

NLRPs (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing Proteins) are members of NLR (Nod-like receptors) protein family. NRLP genes are known to play important roles in both the mammalian reproductive system and the innate immune system. NLRP3 may function as an inducer of apoptosis, activator of NF-kappa-B signaling and activates caspase-1 in response to triggers such as bacterial or viral infection. NLRP3 defects are linked to familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). NLRP3 is highly expressed in blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.

Specificity

Other homologies: Porcine (80% sequence homology).

This antibody recognizes the DAPIN domain of NLRP3.

Immunogen

Epitope: DAPIN domain

KLH-conjugated linear peptide corresponding to the DAPIN domain of human NLRP3.

Application

Detect NLRP3 using this rabbit polyclonal antibody, Anti-NLRP3 Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected NLRP3 in human leukemia bone marrow and human tonsil tissue lysate.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NLRP3 in 10 µg of THP-1 cell lysate.

Target description

~112 kDa and ~75 kDa observed. Five isoforms are known to exist at 118 kDa, 112 kDa, 112 kDa, 106 kDa, and 84 kDa This protein has been observed at ~75 kDa in some cell lysates (Kummer, J. A., et al. (2007). J Histochem Cytochem. 55(5):443-452.). Uncharacterized bands may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
THP-1 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_004886. UniProt accession no.: Q96P20. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NLRP3(114548). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.